| Congresso Brasileiro de Microbiologia 2023 | Resumo: 341-2 | ||||
Resumo:Foodborne diseases, a significant global public health issue, encompass various illnesses caused by consuming certain foods. These diseases occur due to the ingestion of food products contaminated with harmful bacteria, their toxins, or chemicals. One of the challenges in food science is the search for alternative antimicrobial agents to control the spread of pathogens and prevent systemic infections. Antimicrobial peptides (AMPs) are biomolecules with rapid and effective antimicrobial activity against many pathogens, while having a low risk of generating bacterial resistance. However, their specificity relies on a yet undiscovered structure-function relationship. The objective of this research was to evaluate the antibacterial activity of B1CTCu5 analogues against Escherichia coli strains. For this purpose, 30 analogous solid-phase peptides (SPPS) were manually synthesized using the F-moc strategy, characterized by MALDI-TOF, and purified by HPLC. The minimum inhibitory concentration study was conducted using E. coli strains in Müller-Hinton medium, employing a 0.5 McFarland scale at 37°C for 24 hours, and the results were observed with 0.01% resazurin. Structural dynamics through molecular docking was performed with multidrug-resistant enteroaggregative E. coli receptors (MDR-EAEC, O157:H7, and W60). The results confirmed that the new peptide, CRBR-2101 (1.35μg/mL), demonstrated specific activity against E. coli, with non-cytotoxic effects and high affinity for the peptide-receptor in multi-resistant strains. Considering the global public health issue of foodborne diseases and the challenges posed by drug-resistant pathogens, CRBR-2101's potential application in food technology holds great significance. By using CRBR-2101 as an antimicrobial agent, food products could be better protected against contamination, ensuring safer consumption and reducing the risk of foodborne illnesses. Overall, the research on CRBR-2101 highlights its potential as a valuable tool in the fight against drug resistance, offering a new approach to address resistant bacterial infections in the medical and food industries. Palavras-chave: Antimicrobial peptides, Antibacterial resistance, Drug design, Drug discovery, E. coli Agência de fomento:The São Paulo Research Foundation - FAPESP Proc. number 2020/16573-3; 2021/14603-5; 2023/01664-1 | |||||